Juhani is a party member in the role-playing video game Star Wars: Knights of the Old Republic (commonly abbreviated as KOTOR). She was a Cathar female and a Knight in the Jedi Order during the Jedi Civil War. She grew up on the planet Taris after her family fled her home-world during the Mandalorian Wars. After the death of her parents, Juhani was forced into slavery for the debts her parents had incurred in their lifetimes. Jedi Knight Revan eventually came along and freed her, realizing Juhani is Force-sensitive. Juhani then traveled to the Jedi Enclave on Dantooine and began training under Jedi Master Quatra.

Near the end of the Jedi Civil War, Juhani mistakenly believed she had killed her master in a heated moment. This caused her brief stint with the dark side and she hid from the Jedi Council until she was again encountered by Revan. At this point, Revan had lost his memories after a betrayal by the Sith Darth Malak. Revan defeated Juhani in a duel, convincing her to come back to the light side and seek forgiveness. She returned to the Jedi Order, later requesting to accompany Revan and fellow Jedi Bastila Shan in their mission to locate and destroy the Star Forge superweapon.

Bugged coding on the initial release allowed Juhani to be attracted to the player character regardless of gender. In subsequent patches, she reverts to same-sex preferences. She and another female Jedi were also heavily implied to be lovers. This would make Juhani the first known gay character in the Star Wars universe.